Description

UbiQ-058 (TAMRA-Ub-PA) is a potent and specific inhibitor of deubiquitylating enzymes (DUBs). This activity probe is labeled on the N-terminus with a 5-carboxytetramethylrhodamine (TAMRA) dye and can be used for activity profiling experiments and determining DUB inhibitor specificity.

It has three unique capabilities:

• it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses;
• it targets all three major DUB families: UCH, USP and OTU;1 thirdly, the TAMRA label allows detection of DUB labeling by direct in-gel fluorescence. This is a less time-consuming and more sensitive read-out than western-blotting.
• cross-reactivity of antibodies can lead to background labeling, something that is not observed with TAMRA-Ub-PA.