Description

TAMRA-Ub-VME (UbiQ-050) is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs), which is labeled on the N-terminus with a 5-carboxytetramethylrhodamine (TAMRA) dye. This ubiquitin-based activity probe can be used for activity profiling experiments and the control of DUB inhibitor specificity. Whereas the first-generation activity probes (such as HA tagged Ub-VME) require immunoblotting for detection, the second-generation TAMRA-Ub-VME probe allows detection of DUB labeling by in-gel fluorescence.

This direct and more sensitive read-out gives more distinct labeling patterns than can be achieved by immunoblotting. In addition, cross-reactivity of antibodies can lead to background labeling, something that is not observed with TAMRA-Ub-VME. Experimental details can be found in the open-access reference, see product sheet for details [de Jong et al].