target identification and validation
UbiQ can assist your target discovery in the Ubiquitin Proteasome System with our wide range of activity-based probes.
applications
- structural studies
- phenotypic protein profiling on cell lysates for target validation
- chemical proteomics for target identification (i.e., pull down, purification, western blotting, mass spectrometry)
targets
- E1-E2-E3 enzymes – with ourTriple E probes
- all major DUB families (UCH, USP, and OTU) and Ubl proteases – with our activity-based DUB probes
- proteasome – with our activity-based probe UbiQ-018
services
- custom development of advanced reagents, e.g. with substrate context
experience
- UbiQ has developed more than 15 custom probes for a range of customers
- numerous DUBs (e.g. USP4, UCH-L5 and a viral ovarian tumor DUB) have been successfully crystallised with our activity-based probes
- target validation and identification of DUBs, E1 and E2 enzymes in infectious diseases (University Glasgow, University of York, GSK)