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Structural biology of UPS enzymes with activity-based probes

Recently three new structures of ubiquitin related enzymes have been successfully elucidated in complex with an activity-based probe:

Activity-based probes have proven to be efficient tools for structural biology. Over the years a wide range of deubiquitinating and ubiquitinating enzymes have been elucidated using these probes. Here we give you an (in-comprehensive) overview of the most recent crystallizations.
  • E2 enzyme: UBE2D3, probe: Ub-Dha (accession number 5IFR1)
  • DUB MINDY-1, probe: Ub-PA (accession number 5JKN, 5JQS)
  • DUB UCH-L5, probe: Ub-PA (accession number 4UEL, 4UF6)
  • DUB USP4, probe: Ub-PA (accession number 2Y6E)
  • DUB CCHF, probe: Ub-PA (accession number 3ZNH)
  • DUB SARS PLpro, probe: diUb-PA (accession number 5E6J)
  • DUB UCH-L1, probe: diUb-PA (accession number 3KW5)
  • DUB UCH-L3, probe: diUb-PA (accession number 1XD3, 2WDT)
  • DUB MCMV M48, probe: diUb-PA (accession number 2J7Q)
  • E1-E2-E3 enzyme probe

UbiQ-101 : Ub-Dha

UbiQ-105 : Nedd8-Dha

  • 1st generation DUB probes: to target the S1 pocket
    UbiQ-057 : Ub-PA

UbiQ-005 : Ub-VME

UbiQ-108 : Ub-VS

 

  • 3rd generation DUB probes: to target the S1 and S2 pockets
    UbiQ-114 : K63 diUb-PA

 

tailor made
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