DUB assay explorer panel
a panel of 4 deubiquitylating enzyme (DUB) activity assay reagents (25 μg each) prepared by total chemical synthesis
Additional information
| Weight | 0.01 kg |
|---|---|
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement | |
| aliquot size |
€400.00
- Description
- Additional information
- references
Description
UbiQ-L03 is a panel of 4 deubiquitylating enzyme (DUB) activity assay reagents (25 μg each) prepared by total chemical synthesis.
- Ub-AMC (UbiQ-001) is a quenched fluorogenic DUB substrate based on the C-terminal derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC). Cleavage of the amide bond between Gly76 of ubiquitin and the AMC moiety results in an increase in fluorescence at λEx /λEx= 380/460 nm.
- Ub-Rh110Gly (UbiQ-002) is a quenched fluorogenic DUB substrate based on the C-terminal derivatization of ubiquitin with rhodamine110-Gly (Rh110Gly). Cleavage of the amide bond between Gly76 of ubiquitin and Rh110Gly results in an increase in fluorescence at λEx /λEx= 535/485 nm.
- TAMRA-Lys(Ub)-Gly (UbiQ-012) is a fluorescence polarization assay reagent based on a 5- carboxytetramethylrhodamine (TAMRA λEx /λEx= 530/580 nm) modified Lys-Gly sequence that is linked to ubiquitin via a native isopeptide bond with the lysine side-chain.
- Ub-aminoluciferin (UbiQ-036) is a DUB substrate based on the C-terminal derivatization of ubiquitin with 6-aminoluciferin (Luc). Upon cleavage by a DUB, the released 6-aminoluciferin functions as a substrate for luciferase, allowing detection of luminescence as a read-out for DUB-activity.
Additional information
| Weight | 0.01 kg |
|---|---|
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement | |
| aliquot size |
Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938
Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360
Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375
Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387