Biotin-Ahx-Ub-MTC
activity-based probe for deubiquitinating enzymes (DUBs), labeled with an N-terminal biotin and C-terminal methyl hydrazinecarboxylate (MTC)
Additional information
| Weight | N/A |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling |
| target | |
| Standard Size | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| regulatory statement |
€250.00
- Description
- Additional information
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Description
Biotin-Ahx-Ub-MTC (UbiQ-378) is an activity-based probe for deubiquitinating enzymes (DUBs). It is based on ubiquitin with an N-terminal biotin and C-terminal methyl hydrazinecarboxylate (MTC) as electrophile. The MTC group has the unique property that it will react with a DUB active site cysteine similarly as a ubiquitinated protein substrate, forming a tetrahedral transition state in which the oxygen anion is stabilized by the oxyanion hole. A 6-aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities.
Additional information
| Weight | N/A |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling |
| target | |
| Standard Size | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| regulatory statement |