cRh110-Ahx-H2B(113-125) K120(biotin-Ahx-Ub)
an H2B(113-125) peptide which is modified at K120 via a native isopeptide bond with N- terminally biotinylated ubiquitin (biotin-Ahx-Ub); modified on the N-terminus with the dye cRh110 and modified with a C-terminal amide
Additional information
| Weight | 0.005 kg |
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| Applications | |
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| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement | |
| aliquot size |
€300.00
- Description
- Additional information
- references
Description
UbiQ-323 is based on an H2B(113-125) peptide which is modified at K120 via a native isopeptide bond with N- terminally biotinylated ubiquitin (biotin-Ahx-Ub); modified on the N-terminus with the dye cRh110 (5-carboxyrhodamine110, exc= 490 nm, emi= 520 nm) and modified with a C-terminal amide. An 6-aminohexanoic acid (Ahx) linker is used to create extra space between the N-terminus and biotin or dye. It can be used as a substrate for ubiquitin proteases, to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs) and as antigen for immunizations.
Additional information
| Weight | 0.005 kg |
|---|---|
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement | |
| aliquot size |
F. El Oualid et al.
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055