Ac-ISG15prox-VPS
a potent, irreversible and specific activity-based probe for ISG15 proteases
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€350.00
- Description
- Additional information
- references
Description
UbiQ-262 is a potent, irreversible and specific activity-based probe for ISG15 proteases. It is prepared by total chemical synthesis and based on the proximal part of mouse ISG15. To improve stability, Cys77 and Cys144 are replaced by Ser. It contains a C-terminal vinyl pentynyl sulfone (VPS) electrophile which allows for post-labeling modification of cross-linked [UbiQ-237]::[ISG15 protease] complexes by using click chemistry with for example biotin-azide.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |