His6-Ahx-Ahx-SUMO2-VPS
a potent, irreversible and specific activity-based probe for SUMO proteases, labeled with His6 on the N-terminus
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases that is prepared by total chemical synthesis. It contains a C-terminal vinyl pentynyl sulfone (VPS) electrophile (Figure 1) and an N-terminal His6 sequence which allows for sensitive identification or purification by anti-His6 antibodies and/or anti-His6-agarose. The VPS electrophile allows for post-labeling modification of cross-linked UbiQ-237::SENP complexes by using click chemistry with for example biotin-azide.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Albrow et al. Chem Biol 2011, 18, 722.
https://www.ncbi.nlm.nih.gov/pubmed/21700208
Mendes et al. Biochim Biophys Acta – Mol Cell Res 2016, 1863, 139.
https://www.ncbi.nlm.nih.gov/pubmed/26522917