HA-Ahx-Ahx-Ub-VPS
N-terminal tagged activity-based probe for deubiquitinating enzymes (DUBs); contains an electrophile that allows post-labeling modification
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
UbiQ-193 is an activity-based probe for deubiquitinating enzymes (DUBs) based on ubiquitin with a C-terminal electrophilic vinyl pentynyl sulfone (VPS) group. UbiQ-193 contains an N-terminal HA-tag (YPYDVPDYA), which is a peptide sequence derived from the influenza hemagglutinin protein and allows for the sensitive identification or purification by anti-HA antibodies and/or anti-HA-agarose. The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the tag. To eliminate Met1 oxidation, Met1 is replaced by norleucine, a well validated Met mimic. UbiQ-193 can be used for proteomics, activity profiling experiments and determining DUB inhibitor specificity. The VPS electrophile allows for post-labeling modification of covalent DUB::UbiQ-193 complexes by using click chemistry with for example biotin-azide.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055
Borodovsky, A., et al. A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14. EMBO J. 20, 5187-5196 (2001).
http://www.ncbi.nlm.nih.gov/pubmed/11566882
Borodovsky, A., et al. Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 9, 1149-1159 (2002).
http://www.ncbi.nlm.nih.gov/pubmed/12401499
de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887