Biotin-Ahx-H2A(112-129) K119Ub (numbering without Met1)
an H2A(112-129) peptide which is modified at K119* via a native isopeptide bond with ubiquitin and modified on the N-terminus with biotin
(numbering without Met1)
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Western Blot, MS, DUB activity profiling, Target inhibitor profiling |
| target | |
| source | human sequence, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
UbiQ-185 is an H2A(112-129) peptide which is modified at K119* via a native isopeptide bond with ubiquitin (Ub) and modified on the N-terminus with biotin. An aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and H2A peptide for efficient access of biotin binding entities. It can be used as a substrate for ubiquitin proteases, to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs) and as antigen for immunizations.
* numbering without Met1
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Western Blot, MS, DUB activity profiling, Target inhibitor profiling |
| target | |
| source | human sequence, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055