Ub photoLeu73
a crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu)
Additional information
| Weight | 0.1 kg |
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| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€250.00
- Description
- Additional information
- references
Description
UbiQ-154 is a new crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu). Upon irradiation of the diazirine with UV light, a very reactive carbene species is formed. Unspecific binding is minimal for carbene based crosslinking because carbenes are quenched quickly by water, meaning only residues that are very nearby (and thereby contribute to tight binding) will react covalently. Thus any unbound UbiQ-154 will react with water before being able to undergo non-specific reactions with other proteins.
Additional information
| Weight | 0.1 kg |
|---|---|
| aliquot size | |
| bulk size | |
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
(1) Chojnacki et al. Cell Chem Biol 2017, 24, 443.
https://www.ncbi.nlm.nih.gov/pubmed/28330605
(2) Liang et al. Angew Chem Int Ed 2017, 56, 2744.
(3) Zhou et al. Nat Comm 2016, 7, article 10589.
(4) Dubinsky et al. Bioorg Med Chem 2012, 20, 554.