Mca-KKVAPYPME-Dap(Dnp)–NH2
an LF2 peptide based fluorogenic proteasome assay reagent for monitoring 26S proteasome, or ClpP protease activity
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€200.00
- Description
- Additional information
- references
Description
Mca-KKVAPYPME-Dap(Dnp)–NH2 (UbiQ-141) is a fluorogenic reagent for monitoring ATP dependant protease activity like in the 26S proteasome and ClpP protease. Because it is longer than standard tri/tetra peptide based assay reagents, UbiQ-141 is not processed by the 20S CP alone and activating factors are required.
Key Characteristics:
- only processed by activated proteasomes
- exhibits a much more pronounced stimulation of proteasome activity than tetrapeptide based proteasome activity assay reagents
- processed by human and bacterial proteasomes
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
UbiQ protocol P007 _ Proteasome activity profiling with assay reagent UbiQ-141
Jastrab, J. B., et al.
An adenosine triphosphate-independent proteasome activator contributes to the virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci USA. 127, E1763-E1772 (2015), DOI: 10.1073/pnas.1423319112
https://www.pnas.org/doi/10.1073/pnas.1423319112
Smith DM et al.
Docking of the proteasomal ATPases’ carboxyl termini in the 20S proteasome’s alpha ring opens the gate for substrate entry. Mol Cell. 27 731-744 (2007). DOI: 10.1016/j.molcel.2007.06.033
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2083707/
Smith DM et al.
ATP binding to PAN or the 26S ATPases causes association with the 20S proteasome, gate opening, and translocation of unfolded proteins. Mol Cell. 20 687-698 (2005).
https://doi.org/10.1016/j.molcel.2005.10.019
Bai et al.
Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA. 127, E1983-1992 (2015) DOI: 10.1073/pnas.1512094113
https://www.pnas.org/doi/abs/10.1073/pnas.1512094113