His10-SUMO3-Dha
an activity-based probe for the SUMO ligation pathway, based on SUMO3 K11R C47S labeled with His10 on the N-terminus
Additional information
| Weight | 0.005 kg |
|---|---|
| catalogue | |
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€350.00
- Description
- Additional information
- references
Description
UbiQ-124 is an activity-based probe for the SUMO ligation pathway. It is based on the SUMO3 sequence in which the C-terminal Gly has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with the His10 affinity tag and the SUMO3 sequence contains a K11R (to prevent polysumoylation) and C47S mutation. UbiQ-124 is processed in a native manner by SUMO E1, E2 and E3 ligases and during this process, it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzymes, thereby creating a covalent bond.
Additional information
| Weight | 0.005 kg |
|---|---|
| catalogue | |
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Mulder et al., A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature Chem Bio (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108872/