K63 diUb-PA

Name: K63 diUb-PA
SKU: UbiQ-114
Availability: InStock

a potent, irreversible and specific inhibitor based on K63-linked diUb targeting the S2 pocket of deubiquitinating enzymes (DUBs)

productsheet

code UbiQ-114 Category activity-based probes Tags diubiquitin, K63 diUb PA, K63 linkage, PA probe, S2 pocket DUB

product UbiQ-114 Category activity-based probes Tags diubiquitin, K63 diUb PA, K63 linkage, PA probe, S2 pocket DUB

Additional information

Weight	0.005 kg
aliquot size	25 µg
Applications	Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling
target	DUB
source	human sequence, synthetic
shipping	solution
purity	≥90% by RP-HPLC and SDS-PAGE analysis
molecular weight	17.1 kDa by MS
storage	upon arrival powder at −20°C, solution at −80°C.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€300.00

Description
Additional information
references

Description

K63 diU-PA (UbiQ-114) is a potent, irreversible and specific inhibitor based on K63-linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.

This DUB activity based probe can be used for activity profiling experiments and structural studies. Please note the native distance between the proximal and distal Ub is preserved as much as possible. UbiQ-114 has been prepared by total chemical synthesis.

Additional information

Weight	0.005 kg
aliquot size	25 µg
Applications	Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling
target	DUB
source	human sequence, synthetic
shipping	solution
purity	≥90% by RP-HPLC and SDS-PAGE analysis
molecular weight	17.1 kDa by MS
storage	upon arrival powder at −20°C, solution at −80°C.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/16338352

Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23387960