Biotin-Ahx-Ub pThr12
ubiquitin, phosphorylated on Thr12, labeled on the N-terminus with biotin
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | Pull down, Purification, Phenotypic protein profiling, Compound library screening |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€200.00
- Description
- Additional information
- references
Description
UbiQ-093 is ubiquitin that is phosphorylated on Thr12. Phosphoproteomic studies have identified several phosphorylated sites in ubiquitin, among them pThr12. It is labeled on the N-terminus with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. It has been made by total chemical synthesis and is therefore well-defined in terms of biotin and pThr site and incorporation efficiency (100%).
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | Pull down, Purification, Phenotypic protein profiling, Compound library screening |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Bennetzen et al. Mol Cell Proteomics 2010, 9, 1314.
https://www.ncbi.nlm.nih.gov/pubmed/20164059
Bian et al. J Proteomics 2014, 96, 253.
https://www.ncbi.nlm.nih.gov/pubmed/24275569
Kettenbach et al. Sci Signal, 2011, 4, rs5.
https://www.ncbi.nlm.nih.gov/pubmed/21712546
Sharma et al. Cell Rep 2014, 8, 1583.
https://www.ncbi.nlm.nih.gov/pubmed/25159151
Zhou et al. J Proteome Res 2013, 12, 260.
https://www.ncbi.nlm.nih.gov/pubmed/23186163
El Oualid et al. Angew Chem Int Ed 2010, 49, 10149.
https://www.ncbi.nlm.nih.gov/pubmed/21117055