![UbiQ-091](https://ubiqbio.com/wp-content/uploads/2022/04/UbiQ-091.png)
Biotin-Ahx-Ub pSer 65
ubiquitin, phosphorylated on Ser65, labeled on the N-terminus with biotin, activating Parkin E3 ligase mediated ubiquitination
Additional information
Weight | 0.005 kg |
---|---|
aliquot size | |
Applications | Pull down, Purification, Phenotypic protein profiling, Compound library screening |
source | |
shipping | |
purity | |
molecular weight | |
storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
sample preparation | For detailed sample preparation see product sheet. |
regulatory statement |
€200.00
- Description
- Additional information
- references
Description
UbiQ-091 is ubiquitin that is phosphorylated on Ser65, a ubiquitin variant which has been shown to activate Parkin E3 ligase mediated ubiquitination. It is labeled on the N-terminus with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. It has been made by total chemical synthesis and is therefore well-defined in terms of biotin and pSer site and incorporation efficiency (100%).
Additional information
Weight | 0.005 kg |
---|---|
aliquot size | |
Applications | Pull down, Purification, Phenotypic protein profiling, Compound library screening |
source | |
shipping | |
purity | |
molecular weight | |
storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
sample preparation | For detailed sample preparation see product sheet. |
regulatory statement |
Kane et al. J Cell Biol 2014, 205, 143-153.
https://www.ncbi.nlm.nih.gov/pubmed/24751536
Kazlauskaite et al. Biochem J 2014, 460, 127-139.
https://www.ncbi.nlm.nih.gov/pubmed/24660806
Kondapalli et al. Open Biol 2012, 2, 120080.
https://www.ncbi.nlm.nih.gov/pubmed/22724072
Koyano et al. Nature 2014, 510, 162-166.
https://www.ncbi.nlm.nih.gov/pubmed/24784582
V. Sauve and K. Gehring Cell Res 2014, 24, 1025.
https://www.ncbi.nlm.nih.gov/pubmed/24946738
Spratt et al. Nat Commun 2013, 4, 1983.
https://www.ncbi.nlm.nih.gov/pubmed/23770917
Trempe et al. Science 2013, 340, 1451.
https://www.ncbi.nlm.nih.gov/pubmed/23661642
T. Wauer and D. Komander EMBO J 2013, 32, 2099-2112.
https://www.ncbi.nlm.nih.gov/pubmed/23727886
Yamamoto et al. J Biol Chem 2005, 280, 3390-3399.
https://www.ncbi.nlm.nih.gov/pubmed/15557340