Biotin-Ahx-Ub pSer 65

ubiquitin, phosphorylated on Ser65, labeled on the N-terminus with biotin, activating Parkin E3 ligase mediated ubiquitination

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code UbiQ-091 Category Tags ,
product UbiQ-091 Category Tags ,

Additional information

Weight 0.005 kg
aliquot size

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regulatory statement

€200.00

Description

UbiQ-091 is ubiquitin that is phosphorylated on Ser65, a ubiquitin variant which has been shown to activate Parkin E3 ligase mediated ubiquitination. It is labeled on the N-terminus with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. It has been made by total chemical synthesis and is therefore well-defined in terms of biotin and pSer site and incorporation efficiency (100%).

Additional information

Weight 0.005 kg
aliquot size

Applications

, , ,

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.
regulatory statement

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V. Sauve and K. Gehring Cell Res 2014, 24, 1025.
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Spratt et al. Nat Commun 2013, 4, 1983.
https://www.ncbi.nlm.nih.gov/pubmed/23770917

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https://www.ncbi.nlm.nih.gov/pubmed/23661642

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https://www.ncbi.nlm.nih.gov/pubmed/15557340