Biotin-Ahx-Ub
Ubiquitin, labeled on the N-terminus with biotin
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | Pull down, Purification, Phenotypic protein profiling, Compound library screening |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€150.00 – €1,000.00
- Description
- Additional information
- references
Description
UbiQ-090 is (synthetic) ubiquitin labeled on the N-terminus with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ubiquitin protein for efficient access of biotin binding entities. It has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site. UbiQ-090 can be used alone or in combination with unlabeled Ubiquitin.
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | Pull down, Purification, Phenotypic protein profiling, Compound library screening |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055
Wang, T., et al. Biotin-ubiquitin tagging of mammalian proteins in Escherichia coli. Protein Expr. Purif. 30, 140-149 (2003).
http://www.ncbi.nlm.nih.gov/pubmed/12821332