K48 di-Ubiquitin VME

Name: K48 di-Ubiquitin VME
SKU: UbiQ-086
Availability: InStock

activity-based probe for deubiquitinating enzymes (DUBs) based on K48-linked diUb

productsheet

code UbiQ-086 Category activity-based probes Tags diubiquitin, K48 diUb-VME, vinylmethyl ester, VME probe

product UbiQ-086 Category activity-based probes Tags diubiquitin, K48 diUb-VME, vinylmethyl ester, VME probe

Additional information

Weight	0.05 kg
aliquot size	50 µg
bulk size	1 mg
Applications	Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling
target	DUB
source	human, synthetic
shipping	lyophilized powder
purity	≥90% by RP-HPLC and SDS-PAGE analysis
molecular weight	17.107 Da by MS (calc 17.110 Da)
storage	Powder at −20°C, solution at −80°C.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€350.00

Description
Additional information
references

Description

UbiQ-086 is an activity-based probe for deubiquitinating enzymes (DUBs) based on K48-linked diUb. Here Lys48 has been replaced by a diaminobutyric acid residue equipped with a VME type warhead – the Dab(VME) type of structure is a DUB reactive mimic of the native isopeptidic linked Lys(Gly) residue. Please note the native distance between the proximal and distal Ub is preserved as much as possible.

Additional information

Weight	0.05 kg
aliquot size	50 µg
bulk size	1 mg
Applications	Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling
target	DUB
source	human, synthetic
shipping	lyophilized powder
purity	≥90% by RP-HPLC and SDS-PAGE analysis
molecular weight	17.107 Da by MS (calc 17.110 Da)
storage	Powder at −20°C, solution at −80°C.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

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http://www.ncbi.nlm.nih.gov/pubmed/15531586

de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887

Altun, M., et al. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem. Biol. 18, 1401-1412 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22118674

Mulder, M.P., et al. A native chemical ligation handle that enables the synthesis of advanced activity-based probes: diubiquitin as a case study. ChemBiochem 15, 946-949 (2014).
http://www.ncbi.nlm.nih.gov/pubmed/24623714

Haj-Yahya, N., et al. Dehydroalanine-based diubiquitin activity probes. Org. Lett. 16, 540-543 (2014).
http://www.ncbi.nlm.nih.gov/pubmed/24364494

Li, G., et al. Activity-based diubiquitin probes for elucidating the linkage specificity of deubiquitinating enzymes. Chem. Commun. 20, 216-218 (2014).
http://www.ncbi.nlm.nih.gov/pubmed/24225431

Iphöfer, A., et al. Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes. Chembiochem 13, 1416-1420 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22689415

McGouran, J.F., et al. Deubiquitinating enzyme specificity for ubiquitin chain topology profiled by di-ubiquitin activity probes. Chem. Biol. 20, 1447-1455 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/24290882