TAMRA-Ub-PA
a potent and specific inhibitor of deubiquitylating enzymes (DUBs), labeled with a 5-carboxytetramethylrhodamine (TAMRA) dye on the N-terminus
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | human, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€250.00
- Description
- Additional information
- references
Description
UbiQ-058 (TAMRA-Ub-PA) is a potent and specific inhibitor of deubiquitylating enzymes (DUBs). This activity probe is labeled on the N-terminus with a 5-carboxytetramethylrhodamine (TAMRA) dye and can be used for activity profiling experiments and determining DUB inhibitor specificity.
It has three unique capabilities:
- it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses;
- it targets all three major DUB families: UCH, USP and OTU;1 thirdly, the TAMRA label allows detection of DUB labeling by direct in-gel fluorescence. This is a less time-consuming and more sensitive read-out than western-blotting.
- cross-reactivity of antibodies can lead to background labeling, something that is not observed with TAMRA-Ub-PA.
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | human, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Galardy et al.
Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/16338352
Ekkebus et al.
Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23387960