Biotin-Ahx-Ub-VME
activity-based probe for deubiquitinating enzymes (DUBs), labeled with an N-terminal biotin and C-terminal vinyl methyl ester (VME) electrophile.
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C, solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€250.00
- Description
- Additional information
- references
Description
Biotin-Ahx-Ub-VME (UbiQ-054) is an activity-based probe for deubiquitinating enzymes (DUBs). It is based on ubiquitin with an N-terminal biotin and C-terminal vinyl methyl ester (VME) electrophile. A 6-aminohexanoic acid linker introduces extra space between the biotin and Ub for efficient access of biotin binding entities.
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C, solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Misaghi, S., et al. Structure of the Ubiquitin Hydrolase UCH-L3 Complexed with a Suicide Substrate. J. Biol. Chem. 280, 1512-1520 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15531586
de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887