Ub-Ub(26-39)-FP K33 linked

Name: Ub-Ub(26-39)-FP K33 linked
SKU: UbiQ-047
Availability: InStock

a class II ubiquitinated peptide fluorescence polarization assay reagent based on the peptide sequence 26 – 39 of ubiquitin

productsheet

code UbiQ-047 Category activity assay reagents

product UbiQ-047 Category activity assay reagents

Additional information

Weight	0.005 kg
aliquot size	25 µg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human, synthetic
shipping	solution
purity	≥95% by RP-HPLC
molecular weight	10.5 kDa
storage	Store powder and DMSO stock at −20°C, aqueous stocks at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€300.00

Description
Additional information
references

Description

UbiQ-047 is a class II ubiquitinated peptide fluorescence polarization assay reagent based on the peptide sequence 26 – 39 of ubiquitin. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys33 to Ub via a native isopeptide bond. See reference 5 in the product sheet (open access) for full experimental and analytical details.

Additional information

Weight	0.005 kg
aliquot size	25 µg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human, synthetic
shipping	solution
purity	≥95% by RP-HPLC
molecular weight	10.5 kDa
storage	Store powder and DMSO stock at −20°C, aqueous stocks at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387

Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22195557