Ub-yH2AX-Ser140(PO4)-FP
a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein γH2A-X, which is monoubiquitinated on Lys119 and phosphorylated on Ser140
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
UbiQ-039 (Ub-yH2AX-Ser140(PO4)-FP) is a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein γH2A-X, which is monoubiquitinated on Lys119 and phosphorylated on Ser140. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine, a phosphate group on Ser140 and conjugated at Lys119 to Ub via a native isopeptide bond.
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938
Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360
Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375
Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387
–