K13 Ub-PTEN(5-21)-FP
a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 5 – 21 of PTEN, which is monoubiquitinated on Lys13 and Lys289
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | human, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
UbiQ-030 (K13 Ub-PTEN(5-21)-FP) is a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 5 – 21 of PTEN, a tumor suppressor phosphatase which is monoubiquitinated on Lys13 and Lys289. Monoubiquitination of these sites is important for regulation of PTEN mediated tumor suppression and its nuclear import. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys13 to Ub via a native isopeptide bond. See reference 4 (open access) in the product sheet for full experimental and analytical details.
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | human, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938
Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360
Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375
Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387