SUMO2-FP

a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-2 via a native isopeptide bond with the lysine side-chain

productsheet

code UbiQ-021 Category
product UbiQ-021 Category

Additional information

Weight 0.05 kg
aliquot size

Applications

,

target

source

human sequence, semi-synthetic

shipping

purity

>95%

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

300.00

Description

SUMO2-FP (UbiQ-021) is a fluorescence polarization assay reagent for deSUMOylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-2 via a native isopeptide bond with the lysine side-chain.
Typical substrate concentrations range from 10−100 nM. Effective concentrations of deSUMOylating enzymes can range from 0.01-10 nM but depend on specific assay conditions and method of detection.

Additional information

Weight 0.05 kg
aliquot size

Applications

,

target

source

human sequence, semi-synthetic

shipping

purity

>95%

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement