NEDD8-FP
a fluorescence polarization assay reagent for Nedd8 proteases
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | |
| source | human sequence, semi-synthetic |
| shipping | |
| purity | >95% |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€250.00
- Description
- Additional information
- references
Description
Nedd8-FP (UbiQ-019) is a fluorescence polarization assay reagent for Nedd8 proteases, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to Nedd8 via a native isopeptide bond with the lysine side-chain.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | |
| source | human sequence, semi-synthetic |
| shipping | |
| purity | >95% |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
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Tirat et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Analytical Biochem 2005, 343, 244: http://www.ncbi.nlm.nih.gov/pubmed/15963938
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Huang and Aulabaugh, Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 2009, 565, 127: http://www.ncbi.nlm.nih.gov/pubmed/19551360
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Levine et al. Measurement of specific protease activity utilizing fluorescence polarization. Analytical Biochem 1997, 247, 83: http://www.ncbi.nlm.nih.gov/pubmed/9126375
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Geurink et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 2012, 13, 293: http://www.ncbi.nlm.nih.gov/pubmed/22213387