Me4BodipyFL-Ahx3Leu3VS | fluorescent proteasome probe
a fluorescent proteasome activity probe that allows for accurate profiling of proteasomal activity in cell lysates, intact cells, and murine and human patient-derived material, with high sensitivity using SDS-PAGE
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival, powder at +4°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€350.00
- Description
- Additional information
- references
Description
UbiQ-018, Me4BodipyFL-Ahx3Leu3VS is a fluorescent proteasome activity probe that allows for accurate profiling of proteasomal activity in cell lysates, intact cells, and murine and human patient-derived material, with high sensitivity using SDS-PAGE. The probe allows for direct scanning of the gel for fluorescent emission of the distinct proteasomal subunits and circumvents the use of western-blot analysis.
Due to its suitable biochemical and biophysical properties the fluorescent probe can also be used for confocal laser scanning microscopy and flow cytometry-based experiments.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival, powder at +4°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
UbiQ protocol P006 _ Fluorescence-based proteasome activity profiling
Berkers, C.R., et al. Profiling proteasome activity in tissue with fluorescent probes. Mol. Pharm. 739, 739-748 (2007).
http://www.ncbi.nlm.nih.gov/pubmed/17708652
Berkers, C.R., et al. Probing the specificity and activity profiles of the proteasome inhibitors bortezomib and delanzomib. Mol. Pharm. 9, 1126-1136 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22432738