Ub-AMC

a quenched, fluorescent substrate for deubiquitinating enzymes

productsheet

Additional information

Weight 0.05 kg
aliquot size

bulk

,

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

300.00

Description

Ub-AMC (ubiquitin-AMC, UbiQ-001) is a quenched fluorogenic substrate for deubiquitinating enzymes. It is based on ubiquitin that is functionalised with a C-terminal 7-amido-4-methylcoumarin (AMC). Cleavage of the amide bond between the C-terminal Gly and AMC releases the fluorescent AMC dye (exc 380 nm, emi 460 nm).

Additional information

Weight 0.05 kg
aliquot size

bulk

,

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Dang, L.C., et al. Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes. Biochemistry 1998, 37, 1868: http://www.ncbi.nlm.nih.gov/pubmed/9485312