Ub-AMC

Name: Ub-AMC
SKU: UbiQ-001
Availability: InStock

a quenched, fluorescent substrate for ubiquitin proteases

code UbiQ-001 Category activity assay reagents Tags assay reagent, compound library screening, DUB activity assay, ubiquitin

product UbiQ-001 Category activity assay reagents Tags assay reagent, compound library screening, DUB activity assay, ubiquitin

Additional information

Weight	0.05 kg
aliquot size	100 µg
bulk	1 mg, 3 mg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human sequence, synthetic
shipping	lyophilized powder
purity	≥95% by cation-chromatography and RP-HPLC
molecular weight	8.72 kDa
storage	upon arrival powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€300.00

Description
Additional information
references
ubiquitin-AMC

Description

Ub-AMC (ubiquitin-AMC, UbiQ-001) is a quenched fluorogenic substrate for deubiquitinating enzymes. It is based on ubiquitin that is functionalised with a C-terminal 7-amido-4-methylcoumarin (AMC). Cleavage of the amide bond between the C-terminal Gly and AMC releases the fluorescent AMC dye (exc 380 nm, emi 460 nm).

Additional information

Weight	0.05 kg
aliquot size	100 µg
bulk	1 mg, 3 mg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human sequence, synthetic
shipping	lyophilized powder
purity	≥95% by cation-chromatography and RP-HPLC
molecular weight	8.72 kDa
storage	upon arrival powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

Dang, L.C., et al. Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes. Biochemistry. 37, 1868-1879 (1998). http://www.ncbi.nlm.nih.gov/pubmed/9485312

Ubiquitin-AMC (Ub-AMC, UbiQ-001) is a quenched fluorogenic substrate for deubiquitinating enzymes. It is based on ubiquitin functionalised with a C-terminal amino-methylcoumarin (AMC) dye. Cleavage of the amide bond between the C-terminal Gly and AMC releases the fluorescent AMC (exc/emi= 380/460 nm). UbiQ-001 is a well-established reagent for DUB activity profiling.

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