custom ubiquitinated peptides
Our UbiQ-PEP™ service provides monoubiquitinated peptides with a range of modifications to meet your research needs. Peptides up to 20 amino acids in length are site-selectively monoubiquitinated via a native isopeptide link using our Ub ligation technology. [1,2]
applications:
- epitope mapping (e.g. with deubiquitinating enzymes)
- protein structure-function analysis
- pull-down experiments
- structural biology
specifications and available options with UbiQ-PEP™ service
peptide length | up to 20 amino acids | |
| ubiquitinated lysine | site-selectively monoubiquitinated via a native isopeptide link | |
| minimal manufacturing scale | 1 mg | |
| purity | >90% by HPLC | |
| N-terminal modifications | acetyl, biotin, TAMRA, 5-carboxyRh110 | |
| quality control | LC-MS and/or SDS-PAGE | |
| standard delivery time | 4 to 8 weeks |
want to discuss your project and/or receive a quote?
please contact us at sales@ubiqbio.com
literature
- El Oualid et al. “Chemical synthesis of ubiquitin, ubiquitin-based probes, and diubiquitin”. Angew Chem Int Ed. 2010, 49, 10149.
http://www.ncbi.nlm.nih.gov/pubmed/21117055/ - F. El Oualid & H. Ovaa. “Thiolysine and selenolysine building blocks and their use in site- and chemoselective modification of peptides and proteins. 2009. U.S. Patent U58729009. PCT/NL2010/050277, US61/176,616, EP09160430.6
- Fradet-Turcotte et al. “53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark”. Nature 2013, 499, 50.
http://www.ncbi.nlm.nih.gov/pubmed/23760478