Biotin-Ahx-Ub-MTC
activity-based probe for deubiquitinating enzymes (DUBs), labeled with an N-terminal biotin and C-terminal methyl hydrazinecarboxylate (MTC)
Additional information
| Weight | N/A |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling |
| target | |
| Standard Size | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
Biotin-Ahx-Ub-MTC (UbiQ-378) is an activity-based probe for deubiquitinating enzymes (DUBs). It is based on ubiquitin with an N-terminal biotin and C-terminal methyl hydrazinecarboxylate (MTC) as electrophile. The MTC group has the unique property that it will react with a DUB active site cysteine similarly as a ubiquitinated protein substrate, forming a tetrahedral transition state in which the oxygen anion is stabilized by the anion hole. A 6-aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities.
Additional information
| Weight | N/A |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling |
| target | |
| Standard Size | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| regulatory statement |
Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/16338352
de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887
Wrigley, J.D. et al. Enzymatic characterisation of USP7 deubiquitinating activity and inhibition. Cell Biochem. Biophys. 60, 99-111 (2011)
http://www.ncbi.nlm.nih.gov/pubmed/21468692