Biotin-Ahx-SUMO2-PA
an activity-based probe for SUMO proteases, tagged with biotin on the N-terminus
Additional information
| Weight | 0.05 kg |
|---|---|
| catalogue | |
| Custom Aliquot size | |
| wholesale | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival, powder at −20°C; solution at −80°C. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
UbiQ-168 is a mechanism activity-based probe for SUMO proteases. It is based on the human SUMO2 sequence (C48S) in which the C-terminal Gly has been replaced by the propargylamide (PA) electrophile. The N-terminus is labeled with biotin and an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. It has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site.
Additional information
| Weight | 0.05 kg |
|---|---|
| catalogue | |
| Custom Aliquot size | |
| wholesale | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival, powder at −20°C; solution at −80°C. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Albrow et al. Chem Biol 2011, 18, 722.
https://www.ncbi.nlm.nih.gov/pubmed/21700208
Mendes et al. Biochim Biophys Acta – Mol Cell Res 2016, 1863, 139.
https://www.ncbi.nlm.nih.gov/pubmed/26522917
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055
Hewing et al. Nat. Comm. 2018, 9, article number: 1162.