Biotin-Ahx-H2B(113-125) K120Ub
an H2B(113-125) peptide which is modified at K120 via a native isopeptide bond with ubiquitin and modified on the N-terminus with biotin
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | human sequence, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | • dissolve the powder in as little DMSO as possible (e.g. 20 – 40 mg/mL) |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
UbiQ-184 is an H2B(113-125) peptide which is modified at K120 via a native isopeptide bond with ubiquitin (Ub) and modified on the N-terminus with biotin. An aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and H2B peptide for efficient access of biotin binding entities. It can be used as a substrate for ubiquitin proteases, to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs) and as antigen for immunizations.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | human sequence, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | • dissolve the powder in as little DMSO as possible (e.g. 20 – 40 mg/mL) |
| regulatory statement |
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055