Biotin-Ahx-H2B(113-125) K120Ub

Name: Biotin-Ahx-H2B(113-125) K120Ub
SKU: UbiQ-184
Availability: InStock

an H2B(113-125) peptide which is modified at K120 via a native isopeptide bond with ubiquitin and modified on the N-terminus with biotin

productsheet

code UbiQ-184 Category ubiquitinated peptides

product UbiQ-184 Category ubiquitinated peptides

Additional information

Weight	0.05 kg
aliquot size	50 µg
Applications	Crystallization
target	DUB
source	human sequence, synthetic
shipping	lyophilized powder
purity	≥95% by HPLC
molecular weight	10.28 kDa
storage	upon arrival powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation	• dissolve the powder in as little DMSO as possible (e.g. 20 – 40 mg/mL) • add the DMSO stock slowly to milliQ (please note the order of addition) • buffer the aq. solution as desired (e.g. 50 mM HEPES pH 8, 100 mM NaCl) • for example, a final buffered stock of 0.5 mg/mL will contain 1.25 vol% DMSO when prepared from a 40 mg/mL DMSO stock.
regulatory statement	for laboratory research use only, not for use in humans

€300.00

Description
Additional information
references

Description

UbiQ-184 is an H2B(113-125) peptide which is modified at K120 via a native isopeptide bond with ubiquitin (Ub) and modified on the N-terminus with biotin. An aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and H2B peptide for efficient access of biotin binding entities. It can be used as a substrate for ubiquitin proteases, to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs) and as antigen for immunizations.

Additional information

Weight	0.05 kg
aliquot size	50 µg
Applications	Crystallization
target	DUB
source	human sequence, synthetic
shipping	lyophilized powder
purity	≥95% by HPLC
molecular weight	10.28 kDa
storage	upon arrival powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation	• dissolve the powder in as little DMSO as possible (e.g. 20 – 40 mg/mL) • add the DMSO stock slowly to milliQ (please note the order of addition) • buffer the aq. solution as desired (e.g. 50 mM HEPES pH 8, 100 mM NaCl) • for example, a final buffered stock of 0.5 mg/mL will contain 1.25 vol% DMSO when prepared from a 40 mg/mL DMSO stock.
regulatory statement	for laboratory research use only, not for use in humans

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055