Biotin-Ahx-SUMO2-VME
a potent, irreversible and specific activity-based probe for SUMO proteases, labeled with biotin on the N-terminus
Additional information
| Weight | 0.05 kg |
|---|---|
| catalogue | |
| Custom Aliquot size | |
| wholesale | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival, powder at −20°C; solution at −80°C. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€300.00
- Description
- Additional information
- references
Description
UbiQ-156 is an activity-based probe for SUMO proteases. It is based on the human SUMO2 sequence (C48S) in which the C-terminal is equipped with the electrophilic vinyl methyl ester (VME) group. The N-terminus is labeled with biotin and an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and SUMO2 protein for efficient access of biotin binding entities. It has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site.
Additional information
| Weight | 0.05 kg |
|---|---|
| catalogue | |
| Custom Aliquot size | |
| wholesale | |
| Applications | Crystallization, Pull down, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling, Target inhibitor profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival, powder at −20°C; solution at −80°C. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Albrow et al. Chem Biol 2011, 18, 722.
https://www.ncbi.nlm.nih.gov/pubmed/21700208
Mendes et al. Biochim Biophys Acta – Mol Cell Res 2016, 1863, 139.
https://www.ncbi.nlm.nih.gov/pubmed/26522917