His6-3C-MAP1LC3a-Dha
an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively), based on MAP1LC3a C17S labeled with His6 on the N-terminus
Additional information
| Weight | 0.005 kg |
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| aliquot size | |
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€350.00
- Description
- Additional information
- references
Description
UbiQ-158 (His6-3C-MAP1LC3a-Dha) is an activity-based probe for E1 activating enzymes and E2 conjugating enzymes that are involved in autophagy (e.g. ATG7 and ATG3, respectively). UbiQ-158 is based on the MAP1LC3a protein sequence in which the C-terminal Gly has been replaced by a dehydroalanine residue (Dha). The N-terminus is labeled with an His6 affinity tag and a 3C protease cleavage site (QG), Cys17 has been mutated to a Ser residue (S). UbiQ-158 is processed in a native manner by E1-E2-E3 enzymes that recognize MAP1LC3a and during this process it forms an electrophilic intermediate that can react with an active-site Cys residue in the E1-E2-E3 cascade, thereby creating a covalent bond.
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Mulder et al., A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature Chem Bio (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108872/