Biotin-Ahx-Ub(1-75)-Dha-Ub
an activity-based probe based on linear diubiquitin, labeled with biotin on the N-terminus
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€350.00
- Description
- Additional information
- references
Description
Biotin-Ahx-Ub(1-75)-Dha-Ub (UbiQ-121) is an activity-based probe for the deubiquitinating enzyme (DUB) OTULIN. It is based on linear diUb in which the Gly76-Met1 dipeptide is replaced by a dehydroalanine-Met (Dha-Met) dipeptide. The N-terminus of the distal Ub is labeled with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space for efficient access of biotin binding entities. UbiQ-121 has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site. UbiQ-121 reacts with OTULIN in a covalent manner without being cleaved into monoUb, indicating correct positioning of the Dha electrophile for reacting with the nucleophilic active site cysteine of OTULIN.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
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http://www.ncbi.nlm.nih.gov/pubmed/24364494
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http://www.ncbi.nlm.nih.gov/pubmed/24225431
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http://www.ncbi.nlm.nih.gov/pubmed/22689415
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http://www.ncbi.nlm.nih.gov/pubmed/24290882
Weber, A., et al. A Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULIN Cell Chem Biol. 2017 DOI: http://dx.doi.org/10.1016/j.chembiol.2017.08.006
https://www.ncbi.nlm.nih.gov/pubmed/28919039