(Biotin-Ahx-Ub)-(DNP-Ahx-Ub) K48

a native K48-linked di-ubiquitin which contains a biotin on the N-terminus of the proximal ubiquitin and N-(2,4-dinitrophenyl) (DNP) group on the N-terminus of the distal ubiquitin

productsheet

code UbiQ-056 Category
product UbiQ-056 Category

Additional information

Weight 0.05 kg
aliquot size

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.
regulatory statement

€200.00

Description

UbiQ-056 is a native K48-linked di-ubiquitin which contains a biotin on the N-terminus of the proximal ubiquitin and N-(2,4-dinitrophenyl) (DNP) group on the N-terminus of the distal ubiquitin. Both tags are separated from the N-terminus by a 6-aminohexanoic acid (Ahx) linker. The biotin can be used as conjugation site for labeled streptavidin or anti-biotin antibodies. UbiQ-056 is designed for the preparation of various K48-linked diUb conjugates, which can serve for example as DUB (HTRF) assay reagents.

Additional information

Weight 0.05 kg
aliquot size

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.
regulatory statement

F. El Oualid et al.
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

R. A. Horton et al.
Horton, R.A., et al. A substrate for deubiquitinating enzymes based on time-resolved fluorescence resonance energy transfer between terbium and yellow fluorescent protein. Anal. Biochem. 360, 138-143 (2007).
http://www.ncbi.nlm.nih.gov/pubmed/17118327

I. H. Engels et al.
Engels, I.H., et al. A time-resolved fluorescence resonance energy transfer-based assay for DEN1 peptidase activity. Anal. Biochem. 390, 85-87 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19328766