Ub-Ub(56-69)-FP K63 linked

Name: Ub-Ub(56-69)-FP K63 linked
SKU: UbiQ-049
Availability: InStock

a class II ubiquitinated peptide fluorescence polarization assay reagent based on the peptide sequence 56 – 69 of ubiquitin

productsheet

code UbiQ-049 Category activity assay reagents

product UbiQ-049 Category activity assay reagents

Additional information

Weight	0.005 kg
aliquot size	25 µg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human, synthetic
shipping	solution
purity	≥95% by RP-HPLC and SDS-PAGE
molecular weight	10.605 Da by MS
storage	Store powder and DMSO stock at −20°C, aqueous stocks at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation	Prepare a 2 mM stock in DMSO (21 mg/mL). Dilute the 2 mM DMSO stock 20x in milliQ affording a 100 μM stock which can be aliquoted and stored. For assays this 100 μM stock can be diluted for example 1000x in buffer affording a final assay solution of 100 nM, the DMSO concentration during the assay is now 0.01 vol%. The concentration can be verified by comparing the fluorescence intensity with that of a known concentration of TAMRA.
regulatory statement	For laboratory research use only, not for use in humans.

€300.00

Description
Additional information
references

Description

UbiQ-049 is a class II ubiquitinated peptide fluorescence polarization assay reagent based on the peptide sequence 56 – 69 of ubiquitin.
The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys63 to Ub via a native isopeptide bond.
See reference 5 in the product sheet (open access) for full experimental and analytical details.

Additional information

Weight	0.005 kg
aliquot size	25 µg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human, synthetic
shipping	solution
purity	≥95% by RP-HPLC and SDS-PAGE
molecular weight	10.605 Da by MS
storage	Store powder and DMSO stock at −20°C, aqueous stocks at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation	Prepare a 2 mM stock in DMSO (21 mg/mL). Dilute the 2 mM DMSO stock 20x in milliQ affording a 100 μM stock which can be aliquoted and stored. For assays this 100 μM stock can be diluted for example 1000x in buffer affording a final assay solution of 100 nM, the DMSO concentration during the assay is now 0.01 vol%. The concentration can be verified by comparing the fluorescence intensity with that of a known concentration of TAMRA.
regulatory statement	For laboratory research use only, not for use in humans.

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387

Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22195557