Ub-FANCD2-FP

Name: Ub-FANCD2-FP
SKU: UbiQ-029
Availability: InStock

a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 557– 565 of FANCD2, a DNA repair protein which is monoubiquitinated on Lys561

productsheet

code UbiQ-029 Category activity assay reagents Tags assay reagent, FANCD2, ubiquitin

product UbiQ-029 Category activity assay reagents Tags assay reagent, FANCD2, ubiquitin

Additional information

Weight	0.005 kg
aliquot size	25 µg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human, synthetic
shipping	lyophilized powder (purple)
purity	≥95% by RP-HPLC and SDS-PAGE
molecular weight	10002 Da by MS (calc Mw 10002 Da)
storage	−20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€300.00

Description
Additional information
references

Description

UbiQ-029 (Ub-FANCD2-FP) is a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 557– 565 of FANCD2, a DNA repair protein which is monoubiquitinated on Lys561. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys561to ubiquitin via a native isopeptide bond.

Additional information

Weight	0.005 kg
aliquot size	25 µg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human, synthetic
shipping	lyophilized powder (purple)
purity	≥95% by RP-HPLC and SDS-PAGE
molecular weight	10002 Da by MS (calc Mw 10002 Da)
storage	−20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387

Sheng, Y., et al. Molecular recognition of p53 and MDM2 by USP7/HAUSP. Nat. Struct. Mol. Biol. 13, 285-291 (2006).
http://www.ncbi.nlm.nih.gov/pubmed/16474402