SUMO1-FP
a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the lysine side-chain
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | human sequence, semi-synthetic |
| shipping | |
| purity | >95% |
| molecular weight | |
| storage | Solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | upon arrival, powder at −20°C, solution at −80°C. Protect from light and avoid multiple freeze/thaw cycles. |
| regulatory statement |
€300.00
- Description
- Additional information
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Description
SUMO1-FP (UbiQ-020) is a fluorescence polarization assay reagent for de-SUMOylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the lysine side-chain.
A typical substrate concentration is 100 nM. Effective concentrations of deSUMOylating enzymes can range from 0.01-10 nM but depend on specific assay conditions and method of detection.
UbiQ-020 is effectively processed by SENP1.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | human sequence, semi-synthetic |
| shipping | |
| purity | >95% |
| molecular weight | |
| storage | Solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles. |
| sample preparation | upon arrival, powder at −20°C, solution at −80°C. Protect from light and avoid multiple freeze/thaw cycles. |
| regulatory statement |
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