di-ubiquitin explorer panel
a panel of all 8 native linked di-ubiquitin (diUb) conjugates: M1 (i.e. linear), K6, K11, K27, K29, K33, K48 and K63 linked (10 μg each)
Additional information
| Weight | 0.08 kg |
|---|---|
| Applications | |
| target | |
| source | |
| shipping | |
| purity | ≥95% |
| molecular weight | |
| storage | upon arrival, powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement | |
| aliquot size |
€400.00
- Description
- Additional information
- references
Description
UbiQ-L01 is an explorer panel of all 8 native linked di-ubiquitin (diUb) conjugates: M1 (i.e. linear), K6, K11, K27, K29, K33, K48 and K63 linked. The 7 isopeptide linked diUb conjugates have been prepared by chemical ligation, and the linear (M1) diUb conjugate by recombinant expression.
UbiQ-L01 can be used to:
- investigate the linkage specificity of proteases that cleave the (iso)peptide linkage between two ubiquitin proteins.
- investigate mechanism of binding and recognition of proteins that contain ubiquitin-associated domains / or ubiquitin-interacting motifs (UIMs).
Additional information
| Weight | 0.08 kg |
|---|---|
| Applications | |
| target | |
| source | |
| shipping | |
| purity | ≥95% |
| molecular weight | |
| storage | upon arrival, powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement | |
| aliquot size |
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010). http://www.ncbi.nlm.nih.gov/pubmed/21117055
Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22195557
Dikic, I., et al. Ubiquitin-binding domains – from structures to functions. Nat. Rev. Mol. Cell. Biol. 10, 659-671 (2010). http://www.ncbi.nlm.nih.gov/pubmed/19773779
Licchesi, J.D., et al. An ankyrin-repeat ubiquitin-binding domain determines TRABID’s specificity for atypical ubiquitin chains. Nat. Struct. Mol. Biol. 19, 62-71 (2012). http://www.ncbi.nlm.nih.gov/pubmed/22157957