TAMRA-Ub
a fluorescent ubiquitin, labeled with TAMRA on the N-terminus
Additional information
| Weight | 0.1 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | human sequence, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€350.00
- Description
- Additional information
- references
Description
TAMRA-Ub (UbiQ-003) is fluorescent ubiquitin labeled on the N-terminus with TAMRA (5- tetramethylrhodamine, exc 550 nm, emi 590 nm). It has been prepared by total chemical synthesis and allows detection of ubiquitylation by in-gel fluorescence. This direct and more sensitive read-out gives more distinct labeling patterns than immunoblotting and does not suffer from background labeling due to cross-reactivity (as seen sometimes with antibody based detection).
Additional information
| Weight | 0.1 kg |
|---|---|
| aliquot size | |
| Applications | |
| target | |
| source | human sequence, synthetic |
| shipping | |
| purity | |
| molecular weight | |
| storage | |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055
de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887
Smit, J.J. et al. Target specificity of the E3 ligase LUBAC for ubiquitin and NEMO relies on different minimal requirements. J. Biol. Chem. 288, 31728-31737 (2013). http://www.ncbi.nlm.nih.gov/pubmed/24030825