DUB activity-based probe explorer panel

a panel of 10 activity-based probes for deubiquitinating enzymes (DUBs) (10 μg each)

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Weight 0.01 kg
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Description

UbiQ-L02 is a panel of the following 10 activity-based probes for deubiquitinating enzymes (DUBs) (10 μg each).

VME PA
tag code name code name
UbiQ-005 Ub-VME UbiQ-057 Ub-PA
HA UbiQ-035 HA-Ahx-Ahx-Ub-VME UbiQ-078 HA-Ahx-Ahx-Ub-PA
Biotin UbiQ-054 Biotin-Ahx-Ub-VME UbiQ-076 Biotin-Ahx-Ub-PA
TAMRA UbiQ-050 TAMRA-Ub-VME UbiQ-058 TAMRA-Ub-PA
Cy5 UbiQ-071 Cy5-Ub-VME UbiQ-072 Cy5-Ub-PA

 

All probes are prepared by total chemical synthesis. These DUB ABPs are potent, irreversible and specific inhibitors of DUBs which can be used for:

  1. inhibiting hydrolysis of poly-Ub chains on substrate proteins and thus enhancement of poly-Ub chain accumulation
  2. structural biology studies of DUB-Ub complexes
  3. DUB activity profiling experiments
  4. determine DUB inhibitor specificity

Additional information

Weight 0.01 kg
Applications

, , , , , ,

target

source

shipping

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

aliquot size

Misaghi, S., et al. Structure of the Ubiquitin Hydrolase UCH-L3 Complexed with a Suicide Substrate. J. Biol. Chem. 280, 1512-1520 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15531586

de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887

Altun, M., et al. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem. Biol. 18, 1401-1412 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22118674