cRh110-Ahx-H2B(113-125) K120(biotin-Ahx-Ub)

an H2B(113-125) peptide which is modified at K120 via a native isopeptide bond with N- terminally biotinylated ubiquitin (biotin-Ahx-Ub); modified on the N-terminus with the dye cRh110 and modified with a C-terminal amide

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Weight 0.005 kg
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Description

UbiQ-323 is based on an H2B(113-125) peptide which is modified at K120 via a native isopeptide bond with N- terminally biotinylated ubiquitin (biotin-Ahx-Ub); modified on the N-terminus with the dye cRh110 (5-carboxyrhodamine110, exc= 490 nm, emi= 520 nm) and modified with a C-terminal amide. An 6-aminohexanoic acid (Ahx) linker is used to create extra space between the N-terminus and biotin or dye. It can be used as a substrate for ubiquitin proteases, to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs) and as antigen for immunizations.

Additional information

Weight 0.005 kg
Applications

, , ,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

aliquot size

F. El Oualid et al.
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055