hISG15-FP

a fluorescence polarization assay reagent for deISGylating enzymes, based on a TAMRA modified Lys-Gly sequence, linked to human ISG15

productsheet

code UbiQ-287 Category
product UbiQ-287 Category

Additional information

Weight 0.025 kg
aliquot size

Applications

,

target

,

source

human sequence, semi-synthetic

shipping

purity

>95%

molecular weight

storage

sample preparation

For detailed sample preparation, please see product sheet.

regulatory statement

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300.00

Description

UbiQ-287 is a fluorescence polarization assay reagent for deISGylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to human ISG15 via a native isopeptide bond.

Additional information

Weight 0.025 kg
aliquot size

Applications

,

target

,

source

human sequence, semi-synthetic

shipping

purity

>95%

molecular weight

storage

sample preparation

For detailed sample preparation, please see product sheet.

regulatory statement

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387

Basters, A., et al. Molecular characterization of ubiquitin-specific protease 18 reveals substrate specificity for interferon-stimulated gene 15. Febs. J. 281, 1918-1928 (2014).
http://www.ncbi.nlm.nih.gov/pubmed/24533902