Broccoli, cabbage and DUB inhibition

Broccoli, cabbage and DUB inhibition

an application of activity based DUB probes

The consumption of cabbage, broccoli and other cruciferous vegetables is associated with a decreased risk of cancer. Certain isothiocyanates (ITCs) are believed to be responsible for the chemoprotective properties of these vegetables.

Professor Hedstrom and co-workers (Brandeis University) now show in a recent report in Cancer Research (online November 5, 2015) that ITCs inhibit DUBs. The ITCs benzyl isothiocyanate and phenethyl isothiocyanate were found to inhibit USP9x and UCH37 (both associated with tumorigenesis) at physiologically relevant concentrations and time scales. It is also proposed that DUB inhibition can explain why ITCs affect inflammatory and DNA repair processes.

To identify which DUBs are inhibited by the ITCs, a competitive DUB activity profiling was performed using UbiQ’s activity based DUB probes.

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